Accurate Determination of Pesticide Residues in Milk by Sonication-QuEChERS Extraction and LC-LTQ/Orbitrap Mass Spectrometry

A modified, quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction procedure combined with sonication Ultra-High Performance Liquid Chromatography–Orbitrap-Mass Spectrometry (UHPLC–Orbitrap-MS) was developed as a sensitive reliable methodology for the determination of multiclass pesticides in full-fat milk. Different amounts EMR-lipid sorbent were assayed cleanup step order to achieve both acceptably high recoveries low co-extractives final extracts. Accurate mass measurements analyte’s pseudo-molecular ions tandem MS fragmentation used quantify identify target pesticides. Analytical performance characteristics method, such linearity, recovery, precision, limit detection (LOD) quantification (LOQ), matrix effects (ME), expanded uncertainty, have been determined method validation fulfilling all criteria its use validated routine method. The successfully applied real samples (by local farms commercial), revealing presence carbendazim one milk sample at concentration level below maximum residue limits.